一、团队名称
微生物系统工程
二、团队组成
本团队有副教授1名，讲师1名
四、团队介绍
团队研究方向是合成生物学，主要研究内容包括高底盘菌株构建，酶的分子设计与改造，微生态制剂，中草药发酵。研究工作覆盖生物、化工、畜牧等学科交叉领域，在纤维素酶领域具有行业影响力。
团队承担了省级项目2项，近三年研究项目近60万元，发表SCI文章17篇，授权发明专利10项。
四、科研成果
（1）代表性的科研项目：
[1] 发酵工程教育部重点实验室，重点实验室开放课题，1200504，微生态制剂增效纤维素酶的多尺度代谢调控技术，2018-2019，3万元，结题，主持。
[2] 发酵工程教育部重点实验室，重点实验室开放课题，1201002，多结构域纤维素酶的分子建构与催化机理研究，2017-2018，4万元，结题，主持。
[3] 湖北省教育厅, 指导性项目，B2017024， 纤维素内切酶耐受乙醇分子改造与耐受分子机制研究,，2017-2018，0万元，结题，主持。
[4] 湖北工业大学，湖北工业大学博士启动基金，BS2013022,酒糟生产饲料的纤维素酶基因工程菌株构建， 2013-2016 ，4 万元，结题，主持。
[5] 发酵工程教育部重点实验室，重点实验室开放课题，KFJJ201002, 海洋黑曲霉降解水葫芦产耐盐纤维素酶的研究，2010-2013，2 万元，结题，主持。
（2）唯一第一作者或唯一通讯作者发表SCI论文
(1)Dongsheng Xue, Donghai Yao, Xihuo You, Chunjie Gong*. Tandem integration of aerobic fungal cellulase production,lignocellulose substrate saccharification and anaerobic ethanol fermentation by a modified gas lift bioreactor. Bioresource Technology, 2020. . 2020.122902
(2)Dongsheng Xue, Yanming Jiang, Chunjie Gong*. Exogenous xylanase expression simultaneously with the indigenous cellulase to increase the cellulose hydrolysis efficiency.International Biodeterioration & Biodegradation, 2019, 140: 126-132
(3)Dongsheng Xue , Donghai Yao, Xihuo You, Chunjie Gong. Green Synthesis of the Flavor Esters with a Marine Candida parapsilosis Esterase Expressed in Saccharomyces cerevisiae.Indian Journal of Microbiology,60(2):175–181,2020.
(4)Chunjie Gong , Xihuo You, Shuyang Zhang, Dongsheng Xue*. Functional Analysis of a Glutamine Biosynthesis Protein from a Psychrotrophic Bacterium, Cryobacterium soli GCJ02.Indian Journal of Microbiology,60(2):159–152,2020
(5) Chunjie Gong, Qiliang Lai, Hanfeng Cai, Yanming Jiang, Hu Liao, Yunfei Liu , Dongsheng Xue*. Cryobacterium soli sp. nov., isolated from forest soil. International Journal of Systematic and Evolutionary Microbiology, 2020,70:675–679
(6)Dongsheng Xue, Huiying Chen, Yiran Ren, Shanjing Yao*. Enhancing of the activity and thermostability of the thermostable β-glucosidase from a marine Aspergillus niger at high salinity, Process Biochemistry, 47(4) :606-611, 2012.
(7)Dong sheng Xue, Long yuan Liang, Gang Zheng, Dong-qiang Lin, Qi-lei Zhang, Shan-Jing Yao*. Expression of Piromyces rhizinflata cellulase in marine Aspergillus niger to enhance halostable cellulase activity by adjusting enzyme- composition. Biochemical Engineering Journal 117, 156–161, 2016.
(8)Dongsheng Xue, Huiying Chen, Dongqiang Lin, Yixin Guan, Shanjing Yao*. Optimization of a natural medium for cellulase by a marine Aspergillus niger using response surface methodology, Applied Biochemistry and Biotechnology, 167(7): 1963-1972, 2012.
(9)Dongsheng Xue, Jiangbo Wang, Shanjing Yao*. High production of halostable β-glucosidase from a marine Aspergillus nige irmmobilized on towel gourd vegetable sponges. Chinese Chemical Letters, 2015, 25(4):192-196
(10)Dongsheng Xue, Dongqiang Lin, Chunjie Gong, Chunlong Peng, Shanjing Yao . Expression of a bifunctional cellulase with exoglucanase and endoglucanase activities to enhance the hydrolysis ability of cellulase from a marine Aspergillus niger. Process Biochemistry, 52 , 115–122,2016.
(11)Dong sheng Xue, Yuan-long Liang, Dong-qiang Lin, Chun-jie Gong, Shan-Jing Yao. Halostable catalytic properties of exoglucanase from a marine Aspergillus niger and secondary structure change caused by high salinities. Process Biochemistry, 2017, 58：85-91
(12)Longyuan Liang, Dongsheng Xue*. Kinetics of cellulose hydrolysis by halostable cellulase from a marine Aspergillus niger at different salinities. Process Biochemistry，2017, 63：163-168
(13)Dong-sheng Xue, Long-yuan Liang, Dong-qiang Lin, Shan-Jing Yao*. Thermal inactivation kinetics and secondary structure change of a low molecular weight halostable exoglucanase from a marine Aspergillus niger at high salinities. Appl Biochem Biotechnol, 2017,183(3)：1111-1125
(14)Dongsheng Xue, Xuhao Zeng, Dongqiang Lin, Shanjing Yao*.Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii, Chinese Journal of Chemical Engineering, 2018, . 02.007。
(15)Dongsheng Xue, Xuhao Zeng, Dongqiang Lin, Shanjing Yao*. Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii. Chinese Journal of Chemical Engineering
(16)Dongsheng Xue, Xuhao Zeng, Dongqiang Lin, Shanjing Yao*. Ethanol tolerant endoglucanase from Aspergillus niger isolated from wine fermentation cellar. Biocatalysis and Agricultural Biotechnology ,2018,15:19–24
(17)Xuhao Zeng, Dongsheng Xue*. Increasing of activity and thermostability of cold active butanol-tolerant endoglucanase from a marine Rhodococcus sp. under high concentrations of butanol condition. 3 Biotech, 2018,8:265-269.
（3）出版专著
薛栋升，酿酒酶与酶制剂，化学工业出版社，北京，2017，35万字。
（4）授权发明专利
[1] 薛栋升. 一种双基因干扰提高黑曲霉纤维素酶酶活的方法, 2019-11-08, 中国, 201710045642.7.
[2] 薛栋升. 一种大片段基因整合提高黑曲霉纤维素酶酶活的方法，2019-09-10, 中国, 201710250564.4.
[3] 薛栋升. 温度、酸度控制纤维素酶在巨大芽孢杆菌中表达的方法，2019-11-08, 中国, 201610853941.9
[4] 薛栋升. 在缩减基因组的枯草芽孢杆菌中表达黑曲霉葡糖苷酶的方法, 2019-11-08,中国, 201610859539.
[5] 薛栋升, 梁龙元. 增强芽孢杆菌在酸性环境中表达纤维素外切酶效率的方法, 2019-06-25,中国, 201610829645.5
[6] 薛栋升，梁龙元. 一种提高黑曲霉纤维素外切酶在高盐溶液中热稳定性和嗜盐度的方法, 2019-08-20, 中国, 201610559608.7
[7] 薛栋升,, 梁龙元. 利用纤维素内切酶和β-葡糖苷酶构建食品安全级载体及筛选培养基, 2019-10-25, 中国，201610597141.5
[8] 薛栋升,梁龙元. 温度、酸度控制纤维素酶在巨大芽孢杆菌中表达的方法，2019-11-08，中国，201610853941.9
[9] 薛栋升,梁龙元. 一种改变黑曲霉基因组局部结构提高纤维素酶表达量的方法, 2019-11-08, 中国, 201710045636.1.
[10] 薛栋升,梁龙元. 一种磷酸盐诱导启动子降低黑曲霉β-葡萄糖苷酶表达的方法，2019-11-10，中国，201611159365.4
五、联系方式
联系人： 薛栋升 ；宫春杰
邮箱：20121039@hbut.edu.cn （薛栋升）；20141083@hbut.edu.cn（宫春杰）
